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col2a1 #ii–ii 6b3 antibody  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank col2a1 #ii–ii 6b3 antibody
    Histopathological comparisons of young and senescent rat knees: ( a , e ) Young and senescent rat knees, respectively. P: patella; Mu: muscle; F: femur; GC: growth cartilage; LC: lateral condyle; M: medial condyle; M: meniscus; ACL: anterior cruciate ligament; TP: tibial plateau; and T: Tibial. ( b – d , f – h ) Articular cartilage of young and senescent rat knees; respectively. Cho: chondrocytes; SB: spongy bone; CB: compact bone; AC: articular cartilage; CC: calcified cartilage; RL: radial layer; TrL: transitional layer; TL: tangential layer; and Pe: perichondrium. ( a , e ) The images were obtained with a Stereo Zoom Microscope Leica S6D 0.63×. ( b , f ) 15×; ( c , g ) 40×; ( d , h ) 100× and ( k – n ) 15× images were obtained with a Leica DM 2000 LED microscope and a digital camera (Leica MC 170 HD). ( i ) Modified Mankin Score. * p < 0.05. ( j ) Quantification of the number of chondrocytes in both groups, p < 0.05. ( k – m ) Immunohistochemistry of collagen II <t>(Col2a1)</t> and metalloproteinase 13 (Mmp13) in the young and senescent groups, respectively.
    Col2a1 #Ii–Ii 6b3 Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ii+6b3/monoclonal+mouse+antibody+against+collagen+type+ii+ciic1/pmc07697851-138-5-6
    Average 90 stars, based on 1 article reviews
    col2a1 #ii–ii 6b3 antibody - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Histological Evaluation and Gene Expression Profiling of Autophagy-Related Genes for Cartilage of Young and Senescent Rats"

    Article Title: Histological Evaluation and Gene Expression Profiling of Autophagy-Related Genes for Cartilage of Young and Senescent Rats

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms21228607

    Histopathological comparisons of young and senescent rat knees: ( a , e ) Young and senescent rat knees, respectively. P: patella; Mu: muscle; F: femur; GC: growth cartilage; LC: lateral condyle; M: medial condyle; M: meniscus; ACL: anterior cruciate ligament; TP: tibial plateau; and T: Tibial. ( b – d , f – h ) Articular cartilage of young and senescent rat knees; respectively. Cho: chondrocytes; SB: spongy bone; CB: compact bone; AC: articular cartilage; CC: calcified cartilage; RL: radial layer; TrL: transitional layer; TL: tangential layer; and Pe: perichondrium. ( a , e ) The images were obtained with a Stereo Zoom Microscope Leica S6D 0.63×. ( b , f ) 15×; ( c , g ) 40×; ( d , h ) 100× and ( k – n ) 15× images were obtained with a Leica DM 2000 LED microscope and a digital camera (Leica MC 170 HD). ( i ) Modified Mankin Score. * p < 0.05. ( j ) Quantification of the number of chondrocytes in both groups, p < 0.05. ( k – m ) Immunohistochemistry of collagen II (Col2a1) and metalloproteinase 13 (Mmp13) in the young and senescent groups, respectively.
    Figure Legend Snippet: Histopathological comparisons of young and senescent rat knees: ( a , e ) Young and senescent rat knees, respectively. P: patella; Mu: muscle; F: femur; GC: growth cartilage; LC: lateral condyle; M: medial condyle; M: meniscus; ACL: anterior cruciate ligament; TP: tibial plateau; and T: Tibial. ( b – d , f – h ) Articular cartilage of young and senescent rat knees; respectively. Cho: chondrocytes; SB: spongy bone; CB: compact bone; AC: articular cartilage; CC: calcified cartilage; RL: radial layer; TrL: transitional layer; TL: tangential layer; and Pe: perichondrium. ( a , e ) The images were obtained with a Stereo Zoom Microscope Leica S6D 0.63×. ( b , f ) 15×; ( c , g ) 40×; ( d , h ) 100× and ( k – n ) 15× images were obtained with a Leica DM 2000 LED microscope and a digital camera (Leica MC 170 HD). ( i ) Modified Mankin Score. * p < 0.05. ( j ) Quantification of the number of chondrocytes in both groups, p < 0.05. ( k – m ) Immunohistochemistry of collagen II (Col2a1) and metalloproteinase 13 (Mmp13) in the young and senescent groups, respectively.

    Techniques Used: Microscopy, Modification, Immunohistochemistry

    Related Articles

    other:

    Article Title: A new approach to demonstrate cellular activity in bone formation adjacent to implants
    Article Snippet: Bone tissue repeatedly formed in titanium 6–aluminum 4–vanadium rabbit bone harvest implants was collected in vivo at various times between 12 days and 5 weeks.. Qualitative and quantitative examinations on undecalcified thin sections were performed in the light microscope.. The amount of bone tissue was calculated on routinely stained sections.

    Article Title: Flap prefabrication and prelamination with tissue-engineered cartilage.
    Article Snippet: In reconstructive surgery, the integration of tissue-engineered cartilage in a prefabricated free flap may make it possible to generate flaps combining a variety of tissue components, to meet the special requirements of particular defects.. One aim of the present study was to investigate prefabrication of a microvascular free flap by implanting a vessel loop under a skin flap in a rabbit model. A second aim was to report on the authors’ preliminary experiences in prelaminating prefabricated flaps with autologous tissueengineered cartilage, in terms of matrix development, inflammatory reaction, and hosttissue interaction.. The flap was prefabricated by implanting a vessel loop under a random-pattern abdominal skin flap.

    Article Title: Tissue-engineered cartilage using serially passaged articular chondrocytes. Chondrocytes in alginate, combined in vivo with a synthetic (E210) or biologic biodegradable carrier (DBM).
    Article Snippet: In vitro multiplication of isolated autologous chondrocytes is required to obtain an adequate number of cells to generate neo-cartilage, but is known to induce cell-dedi!erentiation.. The aim of this study was to investigate whether multiplied chondrocytes can be used to generate neo-cartilage in vivo.. Adult bovine articular chondrocytes, of various di!erentiation stages, were suspended in alginate at densities of 10 or 50 million/ml, either directly after isolation (P0) or after multiplication in monolayer for one (P1) or three passages (P3).

    Article Title: Improved cartilage integration and interfacial strength after enzymatic treatment in a cartilage transplantation model
    Article Snippet: Antibodies used were M38 and II-6B3 (both 1:100; Developmental Studies Hybridoma Bank) for procollagen type I and collagen type II, respectively; ab6308 (mouse monoclonal IgG antibody, 1:500; Abcam Ltd, Cambridge, UK) for collagen type I; and ab6310 (mouse monoclonal IgG antibody, 1:500, Abcam Ltd) for collagen type III.

    Bioprocessing:

    Article Title: Alginate as a chondrocyte-delivery substance in combination with a non-woven scaffold for cartilage tissue engineering.
    Article Snippet: For tissue engineering of cartilage, chondrocytes can be seeded in a scaffold and stimulated to produce a cartilage-like matrix.. In the present study, we investigated the effect of alginate as a chondrocyte-delivery substance for the construction of cartilage grafts.. E210 (a non-woven fleece of polyglactin) was used as a scaffold.

    Article Title: Minor cartilage collagens type IX and XI are expressed during embryonic stem cell-derived in vitro chondrogenesis.
    Article Snippet: Cartilage development is a complex process that can be analyzed using numerous model systems.. We have previously shown that in vitro differentiation of murine embryonic stem (ES) cells via embryoid bodies (EBs) recapitulates the cellular differentiation steps of chondrogenesis.. However, differentiated chondrocytes lose their characteristic phenotype when they are kept in monolayer culture.

    Incubation:

    Article Title: Dilution of human mesenchymal stem cells with dermal fibroblasts and the effects on in vitro and in vivo osteochondrogenesis
    Article Snippet: The stromal elements of human bone marrow include cells, referred to as mesenchymal stem cells (MSCs), that have the potential to differentiate into bone, cartilage, fat, and hematopoietic-supportive stromal tissue.. MSCs have been isolated and maintained in culture, and in vivo and in vitro assays have been used to show that these cultured cells possess osteochondral potential.. Human mesenchymal stem cells (hMSCs) were combined in a range of proportions with human dermal fibroblasts (hDFs), shown to be devoid of osteochondral potential, and tested in these assays.



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    Histopathological comparisons of young and senescent rat knees: ( a , e ) Young and senescent rat knees, respectively. P: patella; Mu: muscle; F: femur; GC: growth cartilage; LC: lateral condyle; M: medial condyle; M: meniscus; ACL: anterior cruciate ligament; TP: tibial plateau; and T: Tibial. ( b – d , f – h ) Articular cartilage of young and senescent rat knees; respectively. Cho: chondrocytes; SB: spongy bone; CB: compact bone; AC: articular cartilage; CC: calcified cartilage; RL: radial layer; TrL: transitional layer; TL: tangential layer; and Pe: perichondrium. ( a , e ) The images were obtained with a Stereo Zoom Microscope Leica S6D 0.63×. ( b , f ) 15×; ( c , g ) 40×; ( d , h ) 100× and ( k – n ) 15× images were obtained with a Leica DM 2000 LED microscope and a digital camera (Leica MC 170 HD). ( i ) Modified Mankin Score. * p < 0.05. ( j ) Quantification of the number of chondrocytes in both groups, p < 0.05. ( k – m ) Immunohistochemistry of collagen II (Col2a1) and metalloproteinase 13 (Mmp13) in the young and senescent groups, respectively.

    Journal: International Journal of Molecular Sciences

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    Figure Lengend Snippet: Histopathological comparisons of young and senescent rat knees: ( a , e ) Young and senescent rat knees, respectively. P: patella; Mu: muscle; F: femur; GC: growth cartilage; LC: lateral condyle; M: medial condyle; M: meniscus; ACL: anterior cruciate ligament; TP: tibial plateau; and T: Tibial. ( b – d , f – h ) Articular cartilage of young and senescent rat knees; respectively. Cho: chondrocytes; SB: spongy bone; CB: compact bone; AC: articular cartilage; CC: calcified cartilage; RL: radial layer; TrL: transitional layer; TL: tangential layer; and Pe: perichondrium. ( a , e ) The images were obtained with a Stereo Zoom Microscope Leica S6D 0.63×. ( b , f ) 15×; ( c , g ) 40×; ( d , h ) 100× and ( k – n ) 15× images were obtained with a Leica DM 2000 LED microscope and a digital camera (Leica MC 170 HD). ( i ) Modified Mankin Score. * p < 0.05. ( j ) Quantification of the number of chondrocytes in both groups, p < 0.05. ( k – m ) Immunohistochemistry of collagen II (Col2a1) and metalloproteinase 13 (Mmp13) in the young and senescent groups, respectively.

    Article Snippet: In addition, the immunohistochemistry of Col2a1 (DSHB, #II–II 6B3, 0.4 μg/mL in PBS/1% BSA 1:100) and matrix metalloproteinase 13 (Mmp13, ABCAM, ab39012, 1:200) was performed.

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    doi: 10.1016/j.celrep.2023.112012

    Figure Lengend Snippet:

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    Techniques: Virus, Recombinant, Plasmid Preparation, SYBR Green Assay, cDNA Synthesis, DNA HS Assay, Reporter Assay, Software, Imaging

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    Figure Lengend Snippet:

    Article Snippet: Anti-Collagen Type II Antibody, clone 6B3 , Millipore Sigma , Cat# MAB8880, RRID: AB_2260779.

    Techniques: Recombinant, Membrane, Software, Selection, Knock-Out